when to use no and not in a sentence

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I would just like to ask if there are rules of using no and not in a sentence...what hould be the demarcation line in using the two negating words?
 
I would just like to ask if there are rules of using no and not in a sentence...what hould be the demarcation line in using the two negating words?


may be like comma (, ) for instance word : " No, i'm not a doctor " .. CMIIW
 
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